Seven isolates were obtained from typical lesions of angular spot in cucumber and squash leaves obtained from different platations located in the parish of Igarapé, MG. Isolates identify was confirmed through pathogenicity and biochemical tests. Antibiograms indicated insensibility of all isolates to the antibiotics bactitracin and lincomcin up to 1024g/ml, to polymyxin B up to 256g/ml and to antifungal cornpounds cycloheximide, captan, rose bengal and nistatin up to 1024g/ml. The selective mediurn was thought out by adding the afore mentioned antimicrobial substances to medium 523 of Kado & Heskett (1970) as follows: bacitracin, captan, lincomycin, polymyxin B (200g/ml) and cycloheximide, nistatin and rose bengal (300g/ml). The selective medium totally suppressed the growth of several fungi and bacteria in pure cultures but not the growth of P. syringae pv. lachrymans isolates. Moreover, it completely inhibited growth of microrganisms present in a soil suspension or naturally associated to cucumber seeds. The repression index figured out for the selective medium was smaller than 2%. Consequently, the selective medium here developed can be used for detection and quantification of P. syringae pv. lachrymans in substrates like soil, seed extracts and so on.