Seed of Aspidosperma sp., Astronium fraxinifolium, A. urundeuva, Didymopanax marototoni, Terminalia fagifolia, and Virola sebifera were tested for viability and seed health, aining ?ex situ? conservation. The seeds used were obtained from three genetic reserves, two located in Brasilia, DF, and another in the State of Goiás. The germination test was carried out at 20-30°C temperature, using the roll towel technique and a 0,075% tetrazolium salt solution was used for the tetrazolium test. The tetrazolium test and seed germination test showed similar values for the species Aspidosperma sp., Astronium fraxinifolium and A. urundeuva. Seeds of Didymopanax morototoni, Terminalia fagifolia and Virola sebifera did not germinate. To evaluate fungal incidence a seed health test was carried out using the filter paper method. The following fungi, potentially pathogenic, were found: Alternaria sp., Ascochyta sp., Colletotrichum sp., Fusarium sp., Helminthosporium sp., Pestalotia sp., Phoma sp. and Rhizoctonia sp.