THE pH OF EXUDATES-PHENOLPHTALEIN TEST FOR DETERMINING THE CORN SEED QUALITY
DENISE CORREA SANTANA, MARIA DAIS GRAÇAS G.C. VIEIRA,MARIA LAENE MOREIRA DE CARVALHO E MARCELO SÍLVA DE OLIVEIRA
Fast test which determine promptly seed quality have been developed aiming at the
reduction i costs of the production process, minly in the processing packing and storage stages, which
coul be excluded for lots which are below pre determined standards. This work was carried out wit the
objective of determining the temperature, the imbibing time and the type of preparation of th seeds, for
the pH exudates in corn seeds. For the development of the metodology of the p exudates test, mine seed
corn lot is were submitted to the factorial scheme 3 x 4 x 9 (three type of seed preparation: whole,
scarified and sectioned along the axel; four imbibing periods 15, 30, 45 e 60 minutes and nine lots) in a
completely randomized design with eight replications. Two imbibing temperatures were used (25 e
30°C). The pH variation of every seed was measured b the colorimetric method, by addition of one drop
of phenolphtalein at 1% and waterless sodiu carbonate at 0,8g/l to imbibing of the seeds which contained
2ml of destilled and deionized wate to pH = 7,0 ± 0,5. The evaluation was in the terms color tone. The
cells in which the exudate presented rose colour, the seeds were computed as viable light rose as viable
seeds whic originated from abnormal seedlings and colorless as dead seeds. The use of scarified seeds
wit an imbibing time of 30 minutes at a temperature of 25°C, presented a high correlation (r = 0,99 with the
germination test. In the pH exudates test the increase of the imbibing periods of the 30 for 45 e 60
minutes for sectioned seeds under estimate the germination predict of lots.
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