This research was conducted with the objective of evaluating the quality of cotton seed, as measured by germination ability and vigor, of seed collected in different dates from seedcotton arrival at a ginning mill in Uberlândia, M.G., Brazil. Simultaneously, the effects of acid-delinting and fungicide treatment of those seed samples on seed germination were also evaluated. Treatments (three sampling dates; acid delinted vs. fuzzy seed; and fungicide treated vs. untreated seed) were arranged as a factorial array (3x2x2) and the experiment was run as a randomized complete-block design with three replications. Seed quality was evaluated as percent of dead, abnormal, normal and outstanding seedlings obtained in the germination test. Results indicated that : a) seeds with the greatest germination potential were obtained in the first sampling i.e. right after the arrival of seedcotton to the mill, which indicates the deleterious effect of the open storage onto the mill yard; b) from that moment until the ginning itself, there was a reduction of 14.34 and 18.66% on percent germination respectively for acid delinted and fuzzy seed; c) acid delinting promoted better germination rates and higher vigor of seed lots than undelinting; it also considerably reduced the proportion of deteriorated seedlings and seed mortality; d) fungicide treatment efficiently reduced seed deterioration and mortality thereby improving overall seed quality specially for the fuzzy seed samples.