Frequency and the incidence of fungi in soybean seeds vary according to many factors: among those, climatic conditions during the growing season are the most important. The objective of this study was to evaluate the occurrence of the main soybean seedborne pathogens in seeds produced in diverse and most representative soybean growing areas in Brazil from the 1992/93 to 1996/97. Samples of 2051 seed Iots of different cultivars produced in nine localities were assessed for seedborne fungi by the blotter test. Cercospora kikuchii was the most frequent seedborne pathogen in most of the localities, except in Rondonopolis, MT, and Dourados, MS. It was also the pathogen that had the highest average of incidence except in Dourados, MS, and Imperatriz, MA. Fusarium spp. was the most frequent pathogen in Rondonopolis, MT, and Dourados, MS, and the pathogen with the higher mean incidence in Dourados, MS. Phomopsis sp. was the pathogen with the highest incidence average in Imperatriz, MA, and Colletotrichum truncatum was the one with the lowest levels of frequency and average incidence in alI of the localities.