The objective of this work was to verify the influence of the substrate moisture, period of exposure of the seeds in the cold chamber associated to fungicide treatment in the cold test. The experimental design utilized a randomized block, with four replications, in a 4x3x2x2 factorial, involving four corn hybrids (C 333, MR 2,061, C 701 and C 808), three moisture contents in the substrate (50, 60 and 70%), two exposure periods in the cold chamber (five and seven days) and fungicide treatment (with and without treatment). All lots were evaluated for field seedling emergence at three different places (Lavras-MG, Patos de Minas-MG and Santa Helena de Goiás-Go). Sanitary evaluation was through the ?Blotter test?. The results showed that the exposure period and moisture content of the substrate influenced the results of vigor in the cold test. Increased number of normal seedlings was found with the exposition of the seeds for a five day period and with the substrate moisture adjusted to 50 and 60% in cold chamber. Seeds treated with fungicides presented higher vigor when submitted to the cold test.