The enzyme activity (isocitrate-lyase) was evaluated during the germination of soybean seeds (Glycine max (L.) Merrill) from cultivars Doko e CAC-1, after have been submitted to stress conditions of cold test and accelerated aging. In both seed stress conditioning, the aspects presented by the enzymatic activities were very similar, reaching a maximum in the sixth day of germination, being greater in the Doko cultivar. Different seeds stress conditioning did not affect the enzymatic degradation tax from the lipidic stock of the seeds. These data indicate that the enzyme is highly stable in soybean seeds, showing the importance of the glyoxylate cycle in the germinative process.