Variations of the chamber temperatures and seed moisture content could difficult the data interpretation for accelerated aging test. This research was carried out in order to evaluate the effect of initial soybean seed moisture content and two aging chambers on: seed moisture content after aging; variation of temperature and air relative humidity (RH) in the outer and inner chambers and results of accelerated aging test. Four soybean seed lots with initial seed moisture content adjusted for 8%, 10% and 12% were used. The seeds were aged using two chambers: germination chamber (BOD type) and water jacketed chamber. The temperature and RH in the outer and inner chambers were measured using a datalogger record. There was a small variation of temperature and RH for both chambers (BOD and water jacketed), specially in the first six hours of the test. The RH in the inner chambers (gerbox) was always above 90%, independent of the
accelerated aging chamber. For the outer chambers only the water jacketed showed RH above
90%. The germination after seed aging was superior for the BOD chamber. When the three initial
seed moisture contents were compared, the germination was less for 12% seed moisture content.
It can concluded that both chambers (BOD and water jacketed) can be used for soybean seed
aging test.