Aiming to evaluate the storage effects on bean (Phaseolus vulgaris L.) cv. Pérola seeds subjected to hydration-dehydration treatments, this work was carried out at the Seed Testing Laboratory and in plastic tunnels at the Agriculture and Plant Breeding Department of the State University of São Paulo (UNESP), Botucatu-SP. The seeds of cv Pérola were submitted to zero, 6, 12, 24, 36, 48 and 72 hours of hydration. After the hydration treatments the seeds were dried hydrated to the initial water content (controls). The seeds were then stored during 2, 4 and 6 months. The germination and vigor (first germination count) evaluations indicated that the six and 12 hours hydration/dehydration treatments decreased the seeds physiological quality. The lower ion leakage (represented by the solution electrical conductivity) of the stored seeds is due to more tegument impermeability. After two and six months of storage seed hydrated/dehydrated during 72 hours had lower field seedling emergence.