It was made a study to evaluate the efficiency of different culture media in detecting
Xanthomonas campestris pv. campestris (Xcc) in cabbage seeds, the artificial inoculation of
cauliflower seeds with Xcc and its effect on the germination. Four culture media were tested: NA,
NSCAA, BSCAA and SX-Agar. The seeds of cabbage cv. Matsukase suffered previous superficial
desinfestation (sodium hipoclorite 1%, 3min.) and were placed for six hours in bacterial suspension
(108ufc/ml). After that period, the seeds were dry and sowing in the respective media. The
evaluations were made by the 2, 3 and 5 days after the sowing. Seeds of cauliflower hybrid AF-
1192 were willing on plates of Petri containing Xcc cultivated in middle NA, for 48 hours. After
that period, the sowing of 100 seeds were accomplished in culture medium NSCAA, being 50
seeds superficially desinfested. After three days were made the evaluations of the percentage of
infected seeds. At the same time, the standard test of germination was accomplished, with a mixture
of healthy seeds with inoculated one by Xcc, resulting in treatments with 20, 40, 60, 80 and 100%
of infected seeds and a witness. Of the studied media, the best was NSCAA, for presenting speed
and easiness in the identification of the bacterial colonies in relation to the other studied media.
The period of contact of 48 hours of the seeds with the pathogen was enough for its infection and
contaminated seeds with Xcc lose its germination capacity, starting from 20% of contamination.