The goal of this study was to determine the polyacrylamide gel electrophoretic
patterns (electrophoregrams) of the hordeínas and isozymes from five Brazilian barley cultivars:
Embrapa 43, Embrapa 127, Embrapa 128, Embrapa 129 e BR 2. The uniformity (homogeneity) of
these patterns also was determined through the 90 single seed samples of each cultivar. Most of
the cultivars were readily discriminated by hordein electrophoregrams; five barley cultivars
presented four different eletroforegrams of hordeins. Three cultivars gave unique hordein patterns,
whereas the two others (Embrapa 128 e BR 2) presented different esterases (EC 3.1.1.1) patterns.
Jaccard similarity measures of conjugated data of hordeínas and esterases allowed discrimination
of all cultivars and biotypes. The cultivar Embrapa 43 showed two biotypes well defined of hordeins
and esterases. The variation of the hodeins pattern on that cultivar have direct relation with the
variation on zimograms of esterases. This relation was not found in all cultivars that presented
biotypes. It was established that electrophoresis data of hordeins and esterases can be helpful on
barley cultivars identification.