Calendula (Calendula officinalis L.) is a plant with medicinal properties used in
phytotherapic production and in the cosmetic industry. Seed is the basic input, so requirements of
physiological quality to guarantee the establishment of cultivations with high productivity is
essencial. Four lots were used with different origins and storage times of storage to evaluate the
efficiency of methods that allow separation of lots of calendula seeds in vigor levels. The lots were
evaluated using germination test, seed moisture content, seed dry matter weight, weight of 1000
seeds, first count of the germination test, emission of primary root, electrical conductivity, seedling
emergence, speed of seedling emergence, seedling length, seedling green matter and dry matter
weight. The tests of first count of the germination, emission of the primary root, and speed of
seedling emergence index allowed the separation of the lots in vigor levels. The combination of
the information supplied by the comparison of averages and for the correlation analysis between
different vigor test and seedling emergency facilitates better evaluation of the seed quality.