PROCEDURES FOR EVALUATION OF SOYBEAN SEED VIGOR, INCLUDING AN AUTOMATED COMPUTER IMAGING SYSTEM

JULIO MARCOS FILHO, ANA LÚCIA PEREIRA KIKUTI, LIANA BAPTISTA DE LIMA

 

Speed and uniformity of seedling emergence and stand establishment are crucial issues to support high productivity soybean crops. As a consequence, the development and improvement of reproducible procedures to evaluate the physiological attributes of seed quality is a continuing need of the seed industry. Soybean seed vigor has been primarily evaluated by the accelerated aging, electrical conductivity, tetrazolium and seedling growth tests, but there are still opportunities to improve methodology. The objective of this study was to verify the accuracy of an automated system for seedling evaluation (SVIS®) to assess soybean seed vigor in comparison to the information from accepted vigor tests and seedling emergence results. Five seed lots each of two cultivars (Pintado and TMG 113RR) were subjected to standard germination, traditional accelerated aging (41oC and 100% R.H. for 48h followed by germination at 25oC for 4 days), saturated salt accelerated aging - SSAA (41oC and 75% R.H. for 72h), and field seedling emergence. The Seed Vigor Imaging System (SVIS®) parameters comprised mean seedling growth, uniformity and vigor index in images of 3-day old seedlings after germination or SSAA tests. The experimental period comprised three evaluation times at 30-day intervals. Results showed that the information provided by the SVIS® analyses represented a rapid and consistent alternative for soybean seed vigor evaluation with efficiency compared to the accelerated aging test.



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