The objective in this study was establish long-term conservation conditions for Passiflora setacea seeds by testing different seed moisture contents and storage temperatures. Seeds were treated with potassium nitrate (KNO3) and gibberellic acid (GA3) before germination in moistened paper towels. Seed viability was verified by the tetrazolium test after the seeds had been treated. It was observed that: (i) P. setacea seeds can be dried to approximately 4.7% of water content (fresh weight basis) without a significant reduction in germination, (ii) low temperatures and low water content induced dormancy in P. setacea seeds; (iii) dormancy could be overcome by seed treatment with gibberellic acid until the fifth month of storage; (iv) seeds maintained at subzero temperatures showed a higher viability compared to those stored at 4 °C. It was concluded that P. setacea seeds can be stored for up to eight months, at - 20 or -196 °C, since they maintain the minimum international standards for viability.