This study aimed to verify the efficiency of a sequential sampling plan for bean and soybean seeds, in the Sclerotinia sclerotiorum detection. Firstly the heterogeneity of Pérola and Bolinha Bean cultivars lots samples and IACFoscarin soybean was assessed, through H and R testing values, verifying their physiological and sanitary quality. To test the
pathogen presence or absence a positive binomial distribution was used, in naturally infected seeds, and recovered by Neon-S and Roll Paper tests. The Neon-S test efficiency was obtained at different levels of infection. Statistical analysis for each test was made in DIC with four replications and the data subjected to variance analysis, and averages compared using the Tukey test (P ≤ 0.05) probability. The soybean and bean seeds sampling sequence for mycelial Sclerotinia sclerotiorumdetection was efficient, once at least 800 and 1.000 seeds are evaluated considering 0.01 and 0.005%, incidences respectively. The Neon-S and Towel Paper methods were sensitive to the Sclerotinia sclerotiorummycelial detection in soybean and bean seeds.