The pH exudate is a quick test used to check the seed membranes integrity; however, its performance is limited due

to the singularity of each specie requiring adjustments about its methodology. In this sense, the aim of this study was to perform

a quick and accurate physiological potential estimation of Citrus limonia Osb. (‘Rangpur Lime’) seeds through pH exudate

test. First, there was carried out the characterization of five seedlots of ‘Rangpur Lime’ by determination of the thousand seed

mass, water content, germination and emergence. Then, a complete randomized experimental design was installed in a factorial

scheme: 5 × 3 × 2 (five seedlots, three indicator solutions, and two immersion periods), to evaluate the pH exudate. The seeds

were individually distributed in polypropylene trays, and after the immersion periods, there were taken pictures and analyzed by

a numeric scale. Despite the similarity, the emergence test showed to be more efficient for ‘Rangpur Lime’ seeds comparing to

the germination test, because it promoted higher percentage of seedlings. Regarding the pH exudate test, there were not observed

differences between the seedlots for both analysis, visual and image, not being efficient to discriminate lots of ‘Rangpur Lime’.