Effect of reduced water potential on seed germination of a forest tree: a hydrotime approach
Luís Felipe Daibes, Victor J.M. Cardoso
Hydrotime (θH) models provide information on seed tolerance to low water
potential and time to germination under different conditions. Here it was evaluated the
capacity of graphic and probit model to describe germination parameters and germination
times (t) in a tropical legume (Peltophorum dubium). Germination tests were conducted
under reduced water potentials (polyethylene glycol solutions from 0 to -1.2 MPa) at 25
°C. Regression lines were applied to investigate the relationship between germination
rates (1/t) and water potential for different germination percentages (fractions 10, 30, 50
and 70%). Those regressions were used in the graphic model to calculate θH (1/slope) and
determine the base water potential (Ψb) as the point which the line intercepts the x-axis
(G% = 0). In the probit model, germination percentages were transformed to probit units
and plotted against Ψb-values to describe germination response under a single regression
line. Values for θH varied from 1.8 to 2.0 MPa day in both models, and Ψb showed a normal
distribution, as presupposed by the probit model. Predicted germination times (t10 and
t50) mostly fell within observed times, thus showing biological relevance of the models to
describe the effects of water potential on seed germination.
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