Non-destructive and high throughput methods have been developed for seed

quality evaluation. The aim of this study was to relate parameters obtained from the free and

automated analysis of digital radiographs of hybrid melons’ seeds to their seeds’ physiological

potential. Seeds of three hybrid melon (Cucumis melo L.) cultivars from commercial lot samples

were used. Radiographic images of the seeds were obtained, from which area, perimeter,

circularity, relative density, integrated density and seed filling measurements were generated

by means of a macro (PhenoXray) developed for ImageJ® software. After the X-ray test, seed

samples were submitted to the germination test, from which variables related to the physiological

quality of the seeds were obtained. Variability between lots was observed for both physical and

physiological characteristics. Results showed that the use of the PhenoXray macro allows largescale

phenotyping of seed radiographs in a simple, fast, consistent and completely free way. The

methodology is efficient in obtaining morphometric and tissue integrity data of melon seeds and

the generated parameters are closely related to physiological attributes of seed quality.